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Inhibition of nerve growth factor‐induced neurite outgrowth of PC12 cells by a protein kinase inhibitor which does not permeate the cell membrane
Author(s) -
Nagashima Ken,
Nakanishi Satoshi,
Matsuda Yuzuru
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)81165-5
Subject(s) - neurite , nerve growth factor , chemistry , microbiology and biotechnology , membrane , cytotoxicity , biophysics , biochemistry , in vitro , biology , receptor
K‐252a, a protein kinase inhibitor of microbial origin, has proven to be a specific inhibitor of nerve growth factor. In this study, the effects of K‐252b, the 9‐carboxylic acid derivative of K‐252a, on nerve growth factor‐induced neurite outgrowth in PC12 cells was examined. K‐252b is hydrophilic and does not permeate the cell membrane of PC12 cells, whereas K‐252a clearly does. K‐252b is, however, as potent as K‐252a itself in inhibiting the nerve growth factor‐induced neurite outgrowth. These results can be interpreted to suggest that effects of K‐252b may be through surface‐bound/anchored K‐252b‐sensitive molecules on PC12 cells.