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The promoter of Saccharomyces cerevisiae FBA1 gene contains a single positive upstream regulatory element
Author(s) -
Compagno Concetta,
Ranzi Bianca Maria,
Martegani Enzo
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)81160-a
Subject(s) - aldolase a , saccharomyces cerevisiae , gene , open reading frame , biology , transcription (linguistics) , fusion gene , regulatory sequence , gene expression , upstream activating sequence , microbiology and biotechnology , aldolase b , response element , fructose bisphosphate aldolase , promoter , genetics , biochemistry , enzyme , peptide sequence , linguistics , philosophy
The glycolytic enzyme fructose 1,6‐bisphosphate aldolase is encoded by the FBA1 gene of Saccharomyces cerevisiae . Transcription of aldolase gene is not regulated by glucose and high levels of expression have been observed also during growth on nonfermentable carbon source. A FBA1::lacZ gene fusion was constructed and a deletion analysis demonstrated the presence of a unique cis ‐acting positive upstream element (UAS) required for high levels of FBA1 expression. This element is located between positions −550 and −440 upstream of the aldolase open reading frame and it contains sequences known to constitute the binding sites for the multifunctional proteins RAP1 and ABF1 and two TTCC motifs.