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Mitochondrial preproteins en route from the outer membrane to the inner membrane are exposed to the intermembrane space
Author(s) -
Rassow Joachim,
Pfanner Nikolaus
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)81157-4
Subject(s) - intermembrane space , mitochondrial intermembrane space , translocase of the inner membrane , translocase of the outer membrane , inner mitochondrial membrane , mitochondrial carrier , inner membrane , microbiology and biotechnology , chemistry , mitochondrion , atp–adp translocase , bacterial outer membrane , membrane , biophysics , biology , biochemistry , mitochondrial membrane transport protein , escherichia coli , gene
Mitochondrial precursor proteins are known to be imported at sites of close contact between mitochondrial outer and inner membranes. We have identified translocation intermediates exposed to the intermembrane space, including the precursor of the ADP/ATP carrier accumulated at the general insertion site GIP, and the precursor of F 1 ‐ATPase subunitβ accumulated on its import pathway at low levels of ATP. These results suggest that mitochondrial contact sites are not sealed structures, but that polypeptides pass (at least partly) through the intermembrane space on their route from the outer membrane to the inner membrane.