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The nucleotide and deduced amino acid sequences of porcine liver proline‐β‐naphthylamidase
Author(s) -
Matsushima Masashi,
Inoue Hideshi,
Ichinose Masao,
Tsukada Shinko,
Miki Kazumasa,
Kurokawa Kiyoshi,
Takahashi Takayuki,
Takahashi Kenji
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)81147-z
Subject(s) - nucleotide , proline , amino acid , chemistry , biochemistry , biology , gene
A cDNA clone for porcine liver proline‐β‐naphthylamidase was isolated and sequenced. The deduced amino acid sequence of 567 residues was highly homologous with those of carboxylesterases (EC 3.1.1.1) previously reported for other species. In addition, proline‐β‐naphthylamidase purified from porcine liver was shown to have strong activity towards p ‐nitrophenylacetate, a representative substrate for carboxylesterases. These results suggest that proline‐β‐naphthylamidase is identical with carboxylesterase.