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Synchronous free Ca 2+ changes in individual neutrophils stimulated by leukotriene B 4
Author(s) -
Davies E.V.,
Campbell A.K.,
Hallett M.B.
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)81121-n
Subject(s) - chemistry , leukotriene b4 , cytosol , calcium , population , medicine , endocrinology , biochemistry , biology , inflammation , enzyme , environmental health , organic chemistry
Calcium (Ca 2+ ) signals were monitored in individual neutrophils using ratio imaging of fura‐2. In contrast to N ‐formyl‐L‐leucyl‐L‐phenylaianine (f‐met‐leu‐phe), which produced grossly asynchronous Ca 2+ signals with delays in response (up to 60 s), leukotriene B 4 (LTB 4 ) provoked synchronous and immediate elevations in cytosolic free Ca 2+ . Some individual neutrophils which responded immediately to LTB 4 , subsequently displayed delayed Ca 2+ signals in response to f‐met‐leu‐phe. A sub‐population of neutrophils failed to respond to both LTB 4 and f‐met‐leu‐phe. The asynchrony of the Ca 2+ signalling to f‐met‐leu‐phe is not, therefore, an obligatory property of signal transduction in neutraphils.