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cDNA cloning of a human androgen‐induced mRNA exhibiting an early and protein synthesis‐independent induction
Author(s) -
Colletta Anthony A.,
Kealey Terence
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)81120-w
Subject(s) - complementary dna , dihydrotestosterone , androgen , messenger rna , biology , microbiology and biotechnology , androgen receptor , polyadenylation , cloning (programming) , rnf4 , cdna library , clone (java method) , gene , prostate cancer , biochemistry , genetics , hormone , cancer , computer science , programming language
The detailed mechanism of action of androgens remains unknown. We have used an androgen‐dependent human prostate cancer cell line and a subtractive cDNA hybridisation strategy to enrich for androgen‐dependent sequences. This yielded a cDNA clone which exhibits the characteristics of a primary trans ‐activated target for androgens. This androgen‐regulated gene encodes a polyadenylated 4.5 kb mRNA which is induced 30–50‐fold within 3 h of treatment with 10 nM dihydrotestosterone. The induction does not require continued protein synthesis as it is maintained in the presence of protein synthesis inhibitors.