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Calcium influx through ‘L’‐type channels into rat anterior pituitary cells can be modulated in two ways by protein kinase C (PKC‐isoform selectivity of 1,2‐dioctanoyl sn ‐glycerol?)
Author(s) -
MacEwan David J.,
Mitchell Rory
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)81108-k
Subject(s) - protein kinase c , anterior pituitary , diacylglycerol kinase , phorbol ester , phorbol , nimodipine , calcium , chemistry , gene isoform , protein kinase a , voltage dependent calcium channel , medicine , depolarization , biophysics , endocrinology , biochemistry , kinase , biology , hormone , gene
The depolarisation‐induced influx of 45 Ca 2+ into anterior pituitary tissue and GH 3 cells through ‘L’‐type, nimodipine‐sensitive channels was investigated. In anterior pituitary prisms, phorbol esters, activators of protein kinase C, caused an enhancement of K + ‐induced 45 Ca 2+ influx. However, in the GH 3 anterior pituitary cell line, phorbol esters inhibited K + ‐induced 45 Ca 2+ influx. The modulation by phorbol esters in both tissues was stereo‐specific and time‐ and concentration‐dependent. The diacylglycerol analogue, 1,2‐dioctanoyl sn ‐glycerol was able to mimic the phorbol ester‐induced enhancement of calcium influx into anterior pituitary pieces, but was ineffective in GH 3 cell. 1,2‐Diactanoyl sn ‐glycerol may selectively activate an isoform of protein kinase C which is responsible for enhanced ‘L’‐type Ca 2+ ‐channel activity.