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Structural and functional analysis of Trichoderma reesei endoglucanase I expressed in yeast Saccaromyces cerevisiae
Author(s) -
Aho Sirpa
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)81100-m
Subject(s) - trichoderma reesei , cellulase , yeast , saccharomyces cerevisiae , chemistry , biochemistry , microbiology and biotechnology , enzyme , biology
The function of the domains of Trichoderma reesei endoglucanase I (EGI) has been studied. Truncated EGI proteins were expressed from the 3′‐end deleted cDNAs in the yeast Saccharomyces cerevisiae under the control of the ADCI expression cassette. EGI protein was detected by monoclonal antibody EI‐2 and EGI activity as cleared zones around growing colonies on agar plates containing hydroxyethylcellulose (HEC) covalently stained with Ostazin brilliant red (OBR). The results showed that the Thr—Ser‐rich hinge region and the conserved ‘tail’ are not necessary for the efficient synthesis and secretion of EGI in yeast, but the intact core region is necessary for the enzymatic activity.