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2D 1 H NMR studies of oxidized 2(Fe 4 S 4 ) ferredoxin from Clostridium pasteurianum
Author(s) -
Bertini Ivano,
Briganti Fabrizio,
Luchinat Claudio,
Messori Luigi,
Monnanni Roberto,
Scozzafava Andrea,
Vallini Giovanni
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)81082-j
Subject(s) - ferredoxin , geminal , two dimensional nuclear magnetic resonance spectroscopy , chemistry , nuclear magnetic resonance spectroscopy , spectroscopy , paramagnetism , crystallography , metal , proton , nuclear magnetic resonance , physics , stereochemistry , enzyme , organic chemistry , quantum mechanics
Oxidized ferredoxin from Clostridium pastetirianum , containing two Fe 4 S 4 clusters, has been investigated using 2D 1 H NMR spectroscopy at 600 MHz. 2D NMR experiments allowed complete assignment of the sixteen isotropically shifted signals corresponding to the β‐CH 2 protons of the eight metal coordinated cysteines. Geminal connectivities of Cys β‐CH 2 protons were identified through magnitude COSY experiments and confirmed through 2D NOESY experiments. A few additional signals could be assigned to the corresponding α‐CH protons. The importance of 2D experiments to achieve firm assignments of isotropically shifted signals in paramagnetic metalloproteins is stressed.