Premium
Different superstructural features of the light responsive elements of the pea genes rbcS‐3A and rbcS‐3.6
Author(s) -
Cacchione Stefano,
Savino Maria,
Tufillaro Annamaria
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)81080-r
Subject(s) - superstructure , chemistry , biology , physics , crystallography , thermodynamics
Analyses of the DNA sequences, named Light Responsive Elements (LREs), relevant in the photoactivation of the two pea genes rbcS‐3A and rbcS‐3.6 , encoding the same protein but differently expressed, have been carried out, taking advantage of two complementary methods: a theoretical analysis, based on conformational energy calculations, and an experimental evaluation of LREs curvature, derived from gel electrophoretic mobilities of multimers of the LREs and of oligonucleotides corresponding to the three boxes in which they can be dissected. Theoretical and experimental analyses show that the curvature or the rbcS‐3A LRE is larger than that of rbcS‐3.6 LRE, and seems to be correlated with rbcS‐3A higher transcription efficiency.