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Cloning, pharmacological characteristics and expression pattern of the rat GABA A receptor α 4 subunit
Author(s) -
Wisden William,
Herb Anne,
Wieland Heike,
Keinänen Kari,
Lüddens Hartmut,
Seeburg Peter H.
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)81076-k
Subject(s) - cloning (programming) , protein subunit , gamma aminobutyric acid receptor subunit alpha 1 , interleukin 10 receptor, alpha subunit , interleukin 5 receptor alpha subunit , receptor , expression cloning , expression (computer science) , microbiology and biotechnology , biology , chemistry , genetics , g alpha subunit , gene , peptide sequence , computer science , programming language
A cDNA of rat brain encoding the GABA A receptor α 4 subunit has been cloned. Recombinant receptors composed of α 4 , β 2 and γ 2 subunits bind with high affinity the GABA agonist [ 3 H]muscimol and the benzodiazepine ‘alcohol antagonist’ [ 3 H]Ro 15‐4513, but fail to bind benzodiazepine agonists. The α 4 subunit is expressed mainly in the thalamus, as assessed by in situ hybridization histochemistry, and may participate in a major population of thalamic GABA A receptors. The α 4 mRNA is found at lower levels in cortex and caudate putamen, and is rare in cerebellum.