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Detection of Na + /H + exchanger mRNA in human neutrophils and lymphocytes using the polymerase chain reaction
Author(s) -
Quednau Beate,
Rosskopf Dieter,
Weiler-Güttler Hartmut,
Siffert Winfried
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)81074-i
Subject(s) - chemistry , microbiology and biotechnology , messenger rna , polymerase chain reaction , radiochemistry , biochemistry , gene , biology
Using the reverse polymerase chain reaction (RT‐PCR), we have examined the expression of Na + /H + exchanger mRNA in human buffy coat preparations, lymphocytes and neutrophils. Total RNA from all cell types was reverse transcribed specifically and then amplified by PCR. The identity of the PCR products was confirmed by restriction enzyme analysis and hybridization with a specific oligonucleotide probe. The detection or low abundance Na + /H + antiporter specific transcripts by RT‐PCR in different human blood cells ex vivo should facilitate future studies on regulatory and pathophysiological aspects of Na + /H + exchanger mRNA expression in human cells and tissue samples.