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NMR investigation of the interaction of mithramycin A with d(ACCCGGGT) 2
Author(s) -
Keniry Max A.,
Banville Debra L.,
Levenson Corey,
Shafer Richard H.
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)81071-f
Subject(s) - chemistry
The binding of mithramcyin A to d(ACCCGGGT) 2 has been investigated by one‐ and two‐dimensional 1 H NMR spectroscopy. Titration of the drug into the octamer solution results in loss of the oligonucleotide C 2 symmetry at stoichiometric ratios less than 4 drug molecules per duplex. However, at a ratio of 4:1 (drug/duplex), the C 2 symmetry of the oligonucleotide is restored. From these data it is evident that more than one complex forms at ratios less than 4:1 while only one complex predominates at the ratio 4:1. This is the first report of a DNA octamer which binds 4 large drug molecules. These results are compared to those we have recently reported for mithramycin binding to d(ATGCAT) 2 , where only a single, bound complex is observed, with a stoichiometry of 2:1.