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Primary structure of the cDNA 5′‐terminal region encoding the N‐terminal domain of the rabbit muscle α‐actinin subunit
Author(s) -
Jokhadze G.G.,
Oleinikov A.V.,
Alakhov Yu.B.,
Nadirashvili N.Sh.,
Zaalishvili M.M.
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)81067-i
Subject(s) - complementary dna , microbiology and biotechnology , primer (cosmetics) , cdna library , protein subunit , nucleotide , reverse transcriptase , protein primary structure , primer extension , biology , peptide sequence , chemistry , biochemistry , gene , polymerase chain reaction , organic chemistry
A number of cDNA clones have been obtained in summary encoding the N‐terminal domain containing 286 amino acid residues of the rabbit skeletal muscle α‐actinin subunit. α‐Actinin cDNA clones were isolated from specific cDNA libraries using the primer extension method for synthesis of the first cDNA chain. A strong stop signal for AMV reverse transcriptase in the 5′‐terminal region of mRNA of α‐actinin was found. It seems there is a G+C rich region (93% G+C nucleotides) including a continuous sequence of 23 G and C nucleotides encoding 6 glycine residues.