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Distribution of a negative regulator of haematopoietic stem cell proliferation (AcSDKP) and thymosin, β4 in mouse tissues
Author(s) -
Pradelles Philippe,
Frobert Yveline,
Crémi Christophe,
Ivonine Hélène,
Frindel Emilia
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)81062-d
Subject(s) - haematopoiesis , regulator , thymosin , stem cell , induced pluripotent stem cell , microbiology and biotechnology , biology , cell growth , biochemistry , chemistry , embryonic stem cell , gene
A competitive enzyme immunoassay using acetylcholinesterase as tracer for thymosin β4, has been developed. Using this assay and a previously described EIA for AcSDKP, a negative regulator of pluripotent haematopoietic stem cell proliferation, the levels of these two peptides were determined in mouse tissue extracts. The combination of EIAs with different HPLC procedures validated these methods and clearly demonstrated the ubiquity of these peptides in mouse tissues. Similar results are reported for rabbit thymus which suggest different hypotheses for AcSDKP biosynthesis.