Premium
Characterization of a tissue‐type plasminogen activator from porcine urine
Author(s) -
Zacharias Ute,
Will Horst
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)81058-g
Subject(s) - urine , plasminogen activator , urokinase , chemistry , fibrinogen , microbiology and biotechnology , antibody , tissue plasminogen activator , amiloride , endocrinology , medicine , biochemistry , biology , immunology , sodium , organic chemistry
Porcine urine, unlike human urine, does not contain detectable amounts of urokinase‐type plasminogen activator (u‐PA). The plasminogen activator present in porcine urine is of tissue‐type (t‐PA) as identified by the following criteria. (1) Porcine urine PA exhibits an M r of 65 000 similar to the M r of human t‐PA (64‐70 000) but distinct from the M r of human u‐PA (55 000). (2) Antibodies against human t‐PA bind and inhibit crude and purified porcine urine PA, while human u‐PA‐specific antibodies do not react with porcine urine PA. (3) Plasminogen activation by porcine urine PA is markedly stimulated in the presence of fibrinogen fragments. (4) Porcine urine PA activity is not affected by concentration of amiloride substantially suppressing human u‐PA activity.