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A distinct 2,5‐di‐( tert ‐butyl)‐1,4‐benzohydroquinone‐sensitive calcium store in bovine adrenal chromaffin cells
Author(s) -
Robinson Iain M.,
Burgoyne Robert D.
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)81057-f
Subject(s) - chromaffin cell , caffeine , chemistry , stimulation , calcium , fura 2 , agonist , intracellular , angiotensin ii , endocrinology , adrenal medulla , medicine , biophysics , receptor , biochemistry , biology , catecholamine , enzyme , cytosol , organic chemistry
The fluorescent Ca 2+ indicator Fura 2 was used to monitor Ca 2+ release induced by the Ins(1,4,5)P 3 ‐mobilizing agonist angiotensin II (Ag II), caffeine and 2,5‐di‐( tert ‐butyl)‐1,4‐benzohydroquinone (tuBHQ), in intact bovine adrenal chromaffin cells. Under low external Ca 2+ conditions, tuBHQ, Ag II and caffeine elicited Ca 2+ rises, indicating Ca 2+ release from internal stores. Prior addition of Ag II had no noticeable effect on the extent of release of Ca 2+ induced by tuBHQ. Stimulation of the cells with tuBHQ before either Ag II or caffeine, similarly had no effect on Ca 2+ released by these two agonists. It was concluded, therefore, that there is a third intracellular Ca 2+ store in bovine adrenal chromaffin cells, distinct and non‐overlapping, from those sensitive to caffeine or Ins(1,4,5)P 3 ‐Mobilizing agonists.