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Introduction of BAPTA into intact rat submandibular acini inhibits mucin secretion in response to cholinergic and β‐adrenergic agonists
Author(s) -
Mills C.Lloyd,
Dormer R.L,
McPherson M.A
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)81054-c
Subject(s) - secretion , stimulation , bapta , adrenergic , cholinergic , endocrinology , medicine , chemistry , intracellular , calcium in biology , exocytosis , biology , biochemistry , receptor
Incorporation of the calcium chelator BAPTA into isolated, intact rat submandibular acini by hypotonic swelling, resulted in complete inhibition of carbamylcholine, noradrenaline and isoproterenol stimulation of mucin secretion. No effects or intracellular BAPTA on cell viability or β‐adrenergic stimulation of cyclic AMP formation were observed. The data are the first to demonstrate that calcium is necessary for β‐adrenergic stimulation of secretion and suggest that Ca 2+ provides a common link in the triggering of exocytosis.