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cDNA sequence of a Drosophila melanogaster gene, D fur 1, encoding a protein structurally related to the subtilisin‐like proprotein processing enzyme furin
Author(s) -
Roebroek Anton J.M.,
Pauli Ilse G.L.,
Zhang Young,
van de Ven Wim J.M.
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)81052-a
Subject(s) - complementary dna , biology , furin , drosophila melanogaster , cdna library , nucleic acid sequence , peptide sequence , genetics , homology (biology) , gene , microbiology and biotechnology , genomic dna , coding region , sequence analysis , rapid amplification of cdna ends , molecular cloning , biochemistry , enzyme
Screening a genomic library of Drosophila melanogaster DNA with a human fur cDNA probe resulted in the isolation of DNA clones that apparently belonged to two different DNA regions of the Drosophila genome. Subsequently, corresponding Drosophila cDNA clones were isolated. Nucleotide sequence analysis indicated that these cDNA clones originated from two different genes, which were called D fur 1 and D fur 2. From overlapping D fur 1 cDNA clones, a composite cDNA could be constructed and analysis of its nucleotide sequence revealed the coding sequence for a protein of 899 amino acid residues. This protein, designated Dfurin1, exhibited striking sequence homology to human furin and contained the same protein domains except for the cysteine‐rich region. Furthermore, unlike human furin. Dfurin1 possessed an extended amino‐terminal region in which a potential transmembrane anchor was present.