Premium
Treatment of intact hepatocytes with synthetic diacyl glycerols mimics the ability of glucagon to cause the desensitization of adenylate cyclase
Author(s) -
Newlands Christine,
Houslay Miles D.
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)81051-9
Subject(s) - adenylate kinase , cyclase , glucagon , chemistry , glycerol , desensitization (medicine) , glycerol kinase , biochemistry , medicine , endocrinology , enzyme , hormone , biology , receptor
Incubation of intact hepatocytes with either of the synthetic diacyl glycerols 1‐oleoyl‐2‐acetyl glycerol (OAG) or dihexanoyl glycerol (DHG) caused the transient uncoupling of the ability of glucagon to stimulate adenylate cyclase in membranes prepared from those cells. No change occurred in either the activity of the catalytic unit of adenylate cyclase or the coupling of G 5 to adenylate cyclase. Diacyl glycerol action appeared to mimic glucagon‐mediated desensitization of adenylate cyclase, suggesting that protein kinase C activation may provide the molecular trigger for glucagon desensitization.