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The cytochrome chain of mitochondria exhibits variable H + /e − stoichiometry
Author(s) -
Papa S.,
Capitanio N.,
Capitanio G.,
De Nitto E.,
Minuto M.
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)81030-c
Subject(s) - stoichiometry , chemistry , chain (unit) , mitochondrion , physics , crystallography , biochemistry , astronomy
A study is presented of the ←H + /e − stoichiometry for H + pumping by the cytochrome chain in isolated rat liver mitochondria under level‐flow and steady‐state conditions. It is shown that the ←H + /e − stoichiometry for the cytochrome chain varies under the influence of the flow rate and transmembrane ΔμH + . The rate‐dependence is shown to be associated with cytochrome c oxidase, whose ←H + /e − ratio varies from 0 to 1, whilst the ←H + /c − ratio for the span covered by cytochrome c reductase is invariably 2.