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Nucleotide sequence of the secY gene from Lactococcus lactis and identification of conserved regions by comparison of four SecY proteins
Author(s) -
Koivula Teija,
Palva Ilkka,
Hemilä Harri
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)81015-z
Subject(s) - lactococcus lactis , biology , conserved sequence , integral membrane protein , bacillus subtilis , gene , membrane protein , nucleic acid sequence , signal peptide , peptide sequence , transmembrane protein , biochemistry , amino acid , secretory protein , genetics , bacteria , membrane , lactic acid , receptor
SecY is an integral membrane protein which participates in the translocation of proteins through the bacterial cell membrane. We have cloned the secY gene of Lactococcus lactis , and found its deduced protein sequence, 439 amino acids long, to be similar in length to the previously determined SecY proteins of Escherichia coli , Bacillus subtilis and Mycoplasma capricolum . Comparison of the L. lactis SecY to the 3 other SecY proteins revealed 90 conserved amino acid residues (21%). Nearly half of the conserved residues are clustered in 2 of the 10 transmembrane segments, and in 2 of the 6 cytoplasmic regions. Some of the conserved regions are apparently responsible for the interactions of SecY with signal sequences, and the proteins SecE and SecA.