Premium
Probing the secondary quinone (Q B ) environment in photosynthetic bacterial reaction centers by light‐induced FTIR difference spectroscopy
Author(s) -
Breton J.,
Berthomieu C.,
Thibodeau D.L.,
Nabedryk E.
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)81014-y
Subject(s) - quinone , chemistry , photochemistry , fourier transform infrared spectroscopy , spectroscopy , rhodobacter sphaeroides , redox , infrared spectroscopy , photosynthetic reaction centre , photosynthesis , electron transfer , stereochemistry , organic chemistry , optics , physics , biochemistry , quantum mechanics
The photoreduction of the secondary electron acceptor, Q B , has been characterized by light‐induced Fourier transform infrared difference spectroscopy of Rb. sphaeroides and Rp. viridis reaction centers. The reaction centers were supplemented with ubiquinone (UQ 10 or UQ 0 ). The Q B − state was generated either by continuous illumination at very low intensity or by single flash in the presence of redox compounds which rapidly reduce the photooxidized primary electron donor P + . This approach yields spectra free from P and P + contributions making possible the study of the microenvironment of Q B and Q B − . Assignments are proposed for the C O vibration of Q B − and tentatively for the C = O and C = C vibrations of Q B .