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The lac repressor and its N‐terminal headpiece can bind a mini‐operator containing a hairpin loop made of a hexaethylene glycol chain
Author(s) -
Maurizot J.C.,
Chevrie K.,
Durand M.,
Thuong N.T.
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)81012-w
Subject(s) - repressor , loop (graph theory) , chemistry , terminal (telecommunication) , lac repressor , operator (biology) , lac operon , biochemistry , mathematics , gene , computer science , combinatorics , escherichia coli , telecommunications , transcription factor
The binding of the lac repressor and the lac repressor N‐terminal headpiece to a mini‐operator with a hairpin loop made of a hexaethylene glycol chain was investigated using circular dichroism spectroscopy. The Iac repressor's headpiece binds to the modified mini‐operator with the same affinity as to a mini‐operator of the same sequence without the hexaethylene glycol loop. The conformational effect due to the binding is not affected by the presence of the hexaethylene loop. It is also shown that the entire lac repressor binds to this modified mini‐operator inducing a conformational change.