Premium
Stimulation of DNA synthesis in Jurkat cells by synergistic action between adenine and guanine nucleotides
Author(s) -
Tokumitsu Yukiko,
Yanagawa Yoshiki,
Nomura Yasuyuki
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)81007-u
Subject(s) - guanine , jurkat cells , nucleotide , stimulation , dna , dna synthesis , chemistry , biochemistry , biology , genetics , neuroscience , t cell , immune system , gene
P 2 ‐purinoceptor agonists stimulated the DNA synthesis of Jurkat cells via a pathway independent of cAMP and intracellular free calcium. The response was greatly enhanced by the synergistic action between adenine and guanine nucleotides, suggesting that binding sites of these nucleotides are different from each other, and the proliferation is stimulated by a novel interaction between adenine and guanine nucleotide receptors. The stimulatory effects of P 2 ‐agonists on proliferation were completely abolished by cholera toxin and attenuated by pertussis toxin, which suggests that substrates for cholera toxin and pertussis toxin are involved in the proliferative pathways associated with P 2 ‐purinoceptors.