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Tryptase TL 2 in the membrane of human T4 + lymphocytes is a novel binding protein of the V3 domain of HIV‐1 envelope glycoprotein gp120
Author(s) -
Kido Hiroshi,
Fukutomi Aiko,
Katunuma Nobuhiko
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80981-8
Subject(s) - glycoprotein , tryptase , chemistry , microbiology and biotechnology , viral envelope , binding domain , antibody , biochemistry , binding site , biology , immunology , mast cell
A novel membrane‐bound serine esterase in cultured human T4 + lymphocytes, recently purified and named tryptase TL 2 , binds specifically to the external envelope protein gp12O of HIV‐1, interacting with its V3 domain. This binding was selectively blocked by inhibitors of tryptase TL 2 with a GPCR sequence in their reactive site, synthetic peptides corresponding with the sequences of the V3 domains of various HIV‐1 strains with the GPGR sequence, and antibody against tryptase TL 2 , or neutralizing antibody against the V3 domain of HTLV‐III B . These findings suggest that tryptase TL 2 is a binding protein of the V3 domain of HIV‐1 envelope glycoprotein.