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Two α subunits of the G q class of G proteins stimulate phosphoinositide phospholipase C‐β1 activity
Author(s) -
Taylor Stephen J.,
Exton John H.
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80976-a
Subject(s) - phosphoinositide phospholipase c , chemistry , protein subunit , phospholipase c , g protein , class (philosophy) , biochemistry , microbiology and biotechnology , enzyme , biology , signal transduction , computer science , gene , artificial intelligence
Two G protein α subunits were detected in preparations of GTPγS‐dependent, phosphoinositide‐specific phospholipase C‐activating proteins from bovine liver membranes. Partial resolution of the two α subunits, of molecular mass 42 and 43 kDa, was achieved by Mono Q chromatography. Quantitation of the levels of each α subunit and reconstitution assays demonstrated that each possessed stimulatory activity towards the β1 isozyme of phospholipase C. Immunoblot analysis showed that the 42 kDa protein was immunologically related to α q , whereas the 43 kDa protein was related to α 11 , another member of the G q class. The data thus show that two different α subunits of the G q class of G proteins stimulate phospholipase C‐β1 Activity.