Premium
Cloning, overexpression, purification and crystallisation of ribosomal protein L9 from Bacillus stearothermophilus
Author(s) -
Vorgias Constantin E.,
Kingswell Adrian J.,
Dauter Zbigniew,
Wilson Keith S.
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80974-8
Subject(s) - cloning (programming) , ribosomal protein , microbiology and biotechnology , crystallization , ribosomal rna , gene , amino acid , biology , bacillus (shape) , protein crystallization , peptide sequence , chemistry , ribosome , biochemistry , genetics , rna , organic chemistry , computer science , programming language
The cloning, sequencing and overexpression of the gene coding for Bacillus stearothermophilus ribosomal protein L9 is described. The sequence corresponds directly to that presented for the protein itself by classical methods, differing at only a few amino acid positions. The purification and crystallisation of the corresponding L9 protein is presented. The crystals are isomorphous to those described for L9 obtained by conventional methods.