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A mouse brain cDNA encodes a novel protein with the protein kinase C phosphorylation site domain common to MARCKS
Author(s) -
Umekage Tadashi,
Kato Kikuya
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80961-2
Subject(s) - marcks , phosphorylation , complementary dna , protein kinase c , protein kinase a , protein kinase domain , chemistry , microbiology and biotechnology , biology , genetics , gene , mutant
We have isolated a mouse brain cDNA clone encoding a protein of 200 amino acids ( M r , 20165) with partial homology with MARCKS (myristoylated alanine‐rich C‐kinase substrate). Two regions show similarity with MARCKS, one is the kinase C phosphorylation site domain which is supposed to bind calmodulin, and the other is the region near to the N‐terminus, including the consensus sequence of myristoylation. It has a similar amino acid composition to MARCKS, but the content of alanine is not as high. It is distributed throughout the mouse brain, but the pattern is not identical with that of MARCKS. Both proteins may be members of a new protein family involved in coupling the protein kinase C and calmodulin signal transduction systems.