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Photoaffinity labeling of dog pancreas microsomes with 8‐azido‐ATP inhibits association of nascent preprolactin with the signal sequence receptor complex
Author(s) -
Zimmermann Richard,
Zimmermann Maria,
Mayinger Peter,
Klappa Peter
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80949-4
Subject(s) - photoaffinity labeling , microsome , biochemistry , protein subunit , receptor , chemistry , biology , enzyme , gene
Transport of bovine preprolactin into dog pancreas microsomes involves a microsomal protein which is sensitive to photoaffinity labeling with azido‐ATP and which is distinct from the ATP‐binding protein, immunoglobulin heavy chain binding protein. Here we addressed the question of what stage of preprolactin transport is affected. Thus a nascent presecretory protein which is related to preprolactin, termed ppl‐86mer, was employed. Here we show that the nascent preprolactin did not become associated with the α‐subunit of the signal sequence receptor complex after photoaffinity labeling of microsomes with azido‐ATP. Therefore, we conclude that the microsomal protein which is sensitive to photoaffinity labeling with azido‐ATP acts prior to the signal sequence receptor complex.