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Genes encoding peroxisomal enzymes are not necessarily assigned on the same chromosome of an n ‐alkane‐utilizable yeast Candida tropicalis
Author(s) -
Fukuda Yuki,
Atomi Haruyuki,
Kurihara Tatsuo,
Hikida Masaki,
Ueda Mitsuyoshi,
Tanaka Atsuo
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80940-5
Subject(s) - malate synthase , peroxisome , candida tropicalis , thiolase , isocitrate lyase , yeast , biochemistry , biology , enzyme , gene , microbiology and biotechnology , glyoxylate cycle
We have resolved eight chromosomal bands from an n ‐alkane‐assimilating yeast, Candida tropicalis pK 233, by using contour‐clamped homogeneous electric field gel electrophoresis (CHEF). From the results of hybridization of DNA probes of yeast peroxisomal enzymes—catalase, acyl‐CoA oxidase, carnitine acetyltransferase, isocitrate lyase, malate synthase, acetoacetyl‐CoA thiolase, and 3‐ketoacyl‐CoA thiolase — to Southern transfers of CHEF gels, these genes were proven not necessarily to be located on the same chromosome. This fact shows that the genes encoding the enzymes tested were not distributed to be cistronic, although simultaneous and inducible synthesis of peroxisomal enzymes occurred in harmony with the proliferation of peroxisomes, suggesting that their co‐ordinated expression might be mainly regulated by certain trans ‐acting factors.