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Differential expression of GABA A receptor α‐subunits in rat brain during development
Author(s) -
McKernan R.M.,
Cox P.,
Gillard N.P.,
Whiting P.
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80936-w
Subject(s) - polyclonal antibodies , protein subunit , blot , microbiology and biotechnology , receptor , antiserum , gel electrophoresis , biology , cytoplasm , polyacrylamide gel electrophoresis , developmental profile , chemistry , biochemistry , antibody , genetics , gene , endocrinology , enzyme
Unique cytoplasmic loop regions of the α 1 , α 2 , α 3 and α 5 subunits of the GABA A receptor have been expressed in E. coli and used to generate polyclonal antisera specific for these subunits. The antibodies identify proteins by SDS‐polyacrylamide gel electrophoresis and western blotting of molecular size 51 kDa, 53 kDa, 59 kDa and 55 kDa, respectively, which show differential patterns of expression during development. Whereas the α 2 and α 3 subunits are present at early stages, the expression of α 1 and α 5 subunits is low at birth and increases with age. This differential expression could be correlated with previous studies examining the developmental expression of BZ1 and BZ2 benzodiazepine binding sites.