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Determination of the branch location of extra N ‐acetyllactosamine units in sialo N‐linked tetraantennary oligosaccharides
Author(s) -
Hokke Cornelis H.,
Kamerling Johannis P.,
van Dedem Gijs W.K.,
Vliegenthart Johannes F.G.
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80931-r
Subject(s) - chemistry , glycoprotein , stereochemistry , biochemistry
An approach is presented for the determination of the branch location of 1 or 2 extra N ‐acetyllactosamine units in sialo N‐linked carbohydrate chains from glycoproteins. Tetraantennary oligosaccharides containing extra N ‐acetyllactosamine units were digested with endo‐β‐galactosidase, followed by treatment with N ‐acetyl‐β‐glucosaminidase, yielding products which could be analysed by 1 H‐NMR spectroscopy, thereby giving conclusive data about the location of the extra units in the intact structures.