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cDNA sequence and differential expression of the mouse Ca 2+ /calmodulin‐dependent protein kinase IV gene
Author(s) -
Jones David A.,
Glod John,
Wilson-Shaw Donna,
Hahn William E.,
Sikela James M.
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80919-t
Subject(s) - calmodulin , complementary dna , protein kinase a , biology , microbiology and biotechnology , cyclin dependent kinase 2 , map2k7 , gene , peptide sequence , kinase , c raf , cdna library , binding protein , map3k7 , biochemistry , enzyme
We have isolated and sequenced a mouse brain cDNA encoding Ca 2+ /calmodulin‐dependent protein kinase IV. The sequence predicts an acidic protein (pI = 4.56) of 469 amino acids ( M r = 52 627) that contains kinase catalytic and calmodulin‐binding domains. The carboxy region has several primary structural features that suggest it may be a readily cleaved attachment domain. This region is highly charged and hydrophilic and contains several PEST sequences, motifs associated with high turnover proteins. Of the tissues examined, expression of the CaM kinase IV gene is restricted to brain and testis, where transcripts are differentially expressed to produce a kinase in both tissues and a calmodulin‐binding protein, calspermin, in testis that lacks a kinase catalytic domain. Ca 2+ ; Calmodulin; Protein kinase; cDNA; Brain; Testis