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Protein kinase C subtypes in endothelial cells
Author(s) -
Mattila Pirkko
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80914-o
Subject(s) - protein kinase c , blot , microbiology and biotechnology , chromosomal translocation , western blot , endothelial stem cell , biology , northern blot , immunoperoxidase , protein kinase a , kinase , chemistry , immunology , messenger rna , antibody , monoclonal antibody , biochemistry , in vitro , gene
Activation of protein kinase C (PKC) has been linked to the regulation of class II expression on endothelial cells by interferon‐γ (IFN‐γ). PKC subtypes in endothelial cells were analyzed using three different approaches, the immunoperoxidase staining of native and IFN‐γ stimulated cells cultured on chamber slides as well as immuno‐ and Northern blotting. All approaches revealed that of the conventional subtypes. α is the predominant form of PKC in endothelial cells. Even though IFN‐γ is able to induce PKC translocation to particulate fractions, no translocation was detected in histological stainings. Western blot studies as well as mRNA studies revealed that IFN‐γ is unable to increase the total amount of PKC in endothelial cells.