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The formation of 3α‐ and 3β‐acetoxytropanes by Datura stramonium transformed root cultures involves two acetyl‐CoA‐dependent acyltransferases
Author(s) -
Richard J. Robins,
Peter Bachmann,
Trevor Robinson,
M. J. C. Rhodes,
Yasuyuki Yamada
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80887-9
Subject(s) - datura stramonium , acyltransferases , chemistry , biochemistry , biology , botany , biosynthesis , enzyme
Tropine (tropan‐3α‐ol) is an intermediate in the formation of hyoscyamine. An acyltransferase activity that can acetylate tropine using acetyl‐coenzyme A as cosubstrate has been found in transformed root cultures of Datura stramonium . A further acyltransferase activity that acetylates pseudotropine (tropan‐3β‐ol) with acetyl‐coenzyme A is also present. These two activities can be partially resolved by anion‐exchange chromatography, some fractions containing only the pseudotropine‐utilizing activity. The basic properties of these two enzymes are reported and their roles in forming the observed alkaloid spectrum of D. stramonium roots discussed.