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Cloning, sequencing and tissue distribution of a candidate G protein‐coupled receptor from rat pituitary gland
Author(s) -
Karin A. Eidne,
Jelka Zabavnik,
T. Peters,
Shigeru Yoshida,
L. E. Anderson,
Philip L. Taylor
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80876-5
Subject(s) - microbiology and biotechnology , complementary dna , messenger rna , xenopus , biology , receptor , oligonucleotide , transmembrane domain , in situ hybridization , gonadotropin releasing hormone receptor , 5 ht5a receptor , gene , biochemistry , gonadotropin releasing hormone , hormone , luteinizing hormone
A new member of the family of G protein‐coupled receptors has been isolated from a rat pituitary cDNA library by the polymerase chain reaction (PCR) using degenerate oligonucleotide primers. The corresponding protein sequence shows seven transmembrane domains and contains conserved regions of homology characteristic of the G protein‐coupled class of receptors. The novel remptor mRNA is expressed in the brain, pituitary gland and testis, and has been localized by in situ hybridization in discrete regions of the brain. Expression of the receptor mRNA in Xenopus oocytes and in transfected mammalian cells has not yet permitted identification of the corresponding ligand for this receptor.