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Replication of cucumber mosaic virus satellite RNA in vitro by an RNA‐dependent RNA polymerase from virus‐infected tobacco
Author(s) -
Guangyu Wu,
J.M. Kaper,
E.M.J. Jaspars
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80870-9
Subject(s) - rna , cucumber mosaic virus , biology , virology , rna dependent rna polymerase , virus , rna polymerase , polymerase , satellite , rna polymerase i , viral replication , microbiology and biotechnology , gene , plant virus , genetics , engineering , aerospace engineering
An RNA‐dependent RNA polymerase purified from tobacco infected with cucumber mosaic virus catalyses the synthesis of (−) and (+) strands of the viral satellite RNA, CARNA 5, but fails to replicate the satellite RNA of peanut stunt virus (PSV). The enzyme replicates the genomic RNAs of the three principal cucumoviruses CMV. PSV and tomato aspermy virus (TAV) with varying efficiencies. The specificity with which CMV RdRp replicates different sequence‐unrelated RNA templates suggests that the site of their recognition requires secondary or higher level structural organization.