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Some electrospray mass spectrometric evidence for the existence of covalent O ‐acyl enzyme intermediates
Author(s) -
David Ashton,
C.R. Beddell,
David Cooper,
Brian N. Green,
R. Oliver,
Kevin J. Welham
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80867-3
Subject(s) - subtilisin , chemistry , imidazole , proteases , serine , electrospray , covalent bond , enzyme , chymotrypsin , electrospray ionization , stereochemistry , mass spectrometry , organic chemistry , chromatography , trypsin
Electrospray mass spectrometry has been used to measure the masses of the species present in solutions of three serine proteases (α‐chymotrypsin, subtilisin Carlsberg and subtilisin BPN′) before, during and after completion of the hydrolytic reaction with cinnamoyl imidazole and indole acryloyl imidazole. The masses measured during the reaction demonstrated that covalent O ‐acyl enzyme intermediates had been formed.