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Tyrosine‐130 in bullfrog thyroglobulin is a thyroid hormone generating site
Author(s) -
Hiroaki Hayashi,
Yoshihiro Ohmiya,
Shintaro Suzuki
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80860-6
Subject(s) - bullfrog , thyroglobulin , tyrosine , thyroid , chemistry , endopeptidase , biochemistry , endocrinology , lysine , medicine , biology , enzyme , amino acid
Bullfrog thyroglobulin was digested with lysyl endopeptidase, known to be highly specific to cut the C‐terminal side of lysine residue in protein, after reduction and car☐ymethylation. We isolated one peptide which lacked the C‐terminal lysine, and which corresponds to 103–129 of bovine thyroglobulin sequence. Tyrosine 130 in the mammalian thyroglobulin molecule is known to be an iodination site. These findings suggest that tyrosine 130 in frog thyroglobulin is a thyroid hormone generating site.