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Homogeneous solutions of hydrophilic enzymes in nonpolar organic solvents New systems for fundamental studies and biocatalytic transformations
Author(s) -
Vadim V. Mozhaev,
K G Poltevsky,
Vladimir I. Slepnev,
G. A. Badun,
Andrey V. Levashov
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80857-y
Subject(s) - cyclohexane , thermostability , octane , homogeneous , toluene , chemistry , enzyme , organic chemistry , catalysis , enzyme catalysis , cycloalkane , thermodynamics , physics
A typical hydrophilic enzyme, CT, can be dissolved in nonpolar organic solvents ( n ‐octane, cyclohexane and toluene) up to μM concentrations. In the homogeneous solution obtained, the enzyme possesses catalytic activity and enormously high thermostability. It does not lose this activity even after several hours refluxing in octane (126°C) or cyclohexane (81°C).