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Cloning of a cDNA that encodes an invertebrate glutamate receptor subunit
Author(s) -
M L Hutton,
Richard J. Harvey,
E.A. Barnard,
Mark G. Darlison
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80846-u
Subject(s) - cloning (programming) , protein subunit , complementary dna , gamma aminobutyric acid receptor subunit alpha 1 , molecular cloning , glutamate receptor , chemistry , microbiology and biotechnology , biology , receptor , biochemistry , gene , g alpha subunit , computer science , programming language
A full‐length cDNA which encodes a putative glutamate receptor polypeptide was isolated from the pond snail Lymnaea stagnalis , using a short stretch of exonic sequence and two variants of the polymerase chain reaction. In this first comparison of invertebrate and vertebrate glutamate receptor sequences, the mature molluscan polypeptide, which comprises 898 amino acids and has a predicted M r of 100 913, displays between 37% and 46% amino‐acid identity to the rat ionotropic glutamate receptor subunits, GluR1 to GluR6.