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Crystallographic characterization by X‐ray diffraction of the M‐intermediate from the photo‐cycle of bacteriorhodopsin at room temperature
Author(s) -
Masayoshi Nakasako,
Mikio Kataoka,
Yoshiyuki Amemiya,
Fumio Tokunaga
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80837-s
Subject(s) - bacteriorhodopsin , diffraction , chemistry , crystallography , halobacterium , x ray crystallography , lattice constant , halobacteriaceae , arginine , membrane , analytical chemistry (journal) , halobacterium salinarum , optics , amino acid , biochemistry , physics , chromatography
The structure of the M‐intermediate appearing in the photo‐cycle of bacteriorhodopsin was studied with X‐ray diffraction techniques at room temperature. The lifetime of the M‐intermediate was prolonged by treatment with an arginine solution at alkaline pH (Nakasako et al.,FEBS Lett. 254, 211–214). The diffraction profile of membranes which had accumulated the M‐intermediate had small but significant differences in the intensities of Bragg reflections and the lattice constant in comparison with that of membranes having trans ‐bacteriorhodopsin. Diffraction intensities were carefully evaluated and the structural changes during the formation of the intermediate were evaluated with difference Fourier analysis. We could find structural changes around helices G and B.