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Spectrophotometric determination of functional characteristics of protein kinases with coupled enzymatic assay
Author(s) -
Zusana Technikova-Dobrova,
Anna Maria Sardanelli,
Sergio Papa
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80836-r
Subject(s) - kinase , enzyme , chemistry , biochemistry , protein kinase a
The performance of enzyme‐coupled spectrophotometric assay of protein kinases and its merits as compared with the radioisotopic method in characterizing functional activity of protein kinases are described. Kinetic parameters of protein kinase C and protein kinase A so obtained are presented. General application of the spectrophotometric assay in the study of protein kinases is recommended.