Premium
Involvement of α‐ and β‐PKC in the differentiation of 3T3‐L1 cells
Author(s) -
Hiroo Ueda,
Yasuyuki Ishida,
Hiroshi Taniguchi
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80833-o
Subject(s) - staurosporine , protein kinase c , 3t3 l1 , 3t3 cells , chemistry , protein kinase a , enzyme , endocrinology , adipose tissue , microbiology and biotechnology , biology , adipocyte , biochemistry , transfection , gene
Present investigation revealed that 3T3‐L1 cells contained two protein kinase C (PKC) subspecies, i.e. α‐ and β‐PKC. These cells treated by staurosporine, a specific inhibitor of PKC, differentiated to adipocytes more remarkably than those without its treatment. Their α‐ and β‐PKC activity decreased to 49% and 18% of the staurosporine‐untreated cells respectively, and GPDH activity, a marker enzyme of adipocytes, increased to 143%. Thus, both α‐ and β‐PKC seemed to be associated with the adipose conversion in 3T3‐L1 cells.