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Solubilization and partial purification of squalene synthase from daffodil microsomal membranes
Author(s) -
Lionel Belingheri,
Peter Beyer,
Hans Kleinig,
Michel Gleizes
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80827-p
Subject(s) - chaps , squalene , microsome , chemistry , solubilization , chromatography , sepharose , atp synthase , enzyme , fraction (chemistry) , biochemistry , membrane
A squalene synthase was solubilized from daffodil ( Narcissus pseudonarcissus L .) microsomes with CHAPS, a zwitterionic non‐denaturating detergent. By successive chromatography on DEAE Sephacel and APP Sepharose a fraction enriched in this enzyme (21‐fold) was prepared.