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Expression of 25‐hydroxyvitamin D 24‐hydroxylase cytochrome P450 in kidney and intestine Effect of 1,25‐dihydroxyvitamin D and age
Author(s) -
H. J. Armbrecht,
M. A. Boltz
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80823-l
Subject(s) - endocrinology , medicine , cytochrome p450 , kidney , messenger rna , biology , hormone , enzyme , chemistry , metabolism , biochemistry , gene
To study the mechanism of hormonal regulation of the 25‐hydroxyvitamin D 24‐hydroxylase, a DNA probe complementary to the published sequence of the recently cloned P450 component [(1991) FEBS Lett. 278, 195] was employed. Young (2 month) and adult (12 month) F344 rats, deficient in 1,25‐dihydroxyvitamin D, were given a single dose or 1,25‐dihydroxyvitamin D. In young rats, 1,25‐dihydroxyvitamin D markedly increased P450 mRNA levels within 3 h in both kidney and intestine, and maximal levels were attained at 16 and 3 h, respectively. In adult animals, maximal induction of mRNA was diminished in the kidney, and the decline was slower in the intestine. Time of maximal induction did not change with age. These studies demonstrate for the first time regulation of the 24‐hydroxylase enzyme by 1,25‐dihydroxyvitamin D at the level ofthe mRNA for the cytochrome P450. They also demonstrate that this regulation may change with age.