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Transcriptional analysis of two Rhodobacter capsulatus ferredoxins by translational fusion to Escherichia coli lacZ
Author(s) -
Y Suetsugu,
Kazuhiko Saeki,
Hiroshi Matsubara
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80822-k
Subject(s) - ferredoxin , rhodobacter , lac operon , escherichia coli , plasmid , fusion protein , transcription (linguistics) , gene , fusion gene , microbiology and biotechnology , biology , chemistry , biochemistry , recombinant dna , enzyme , mutant , linguistics , philosophy
Plasmids which contained the translational fusion of Escherichia coli lacZ to Rhodobacter capsulatus ferredoxin genes, fdxN and fdxA , were constructed. Effects of growth conditions on the expression of each ferredoxin were analyzed by measuring the β‐galactosidase activity in R. capsulatus which harbored a corresponding plasmid. Transcription fdxN :: lacZ , the ferredoxin I fusion gene, was regulated at least 100‐fold by either NH + 4 or O 2 but not by illumination, confirming that fdxN belongs to the nif ‐gene family. Transcription of fdxA :: lacZ , the ferredoxin II fusion gene, however, was constant under all the conditions surveyed, suggesting that the protein has some constitutive function(s).