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Developmental expression of troponin I isoforms in fetal human heart
Author(s) -
Pankaj Bhavsar,
Gurtej K. Dhoot,
D. V. E. Cumming,
Gillian Butler-Browne,
Magdi H. Yacoub,
Paul J.R. Barton
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80820-s
Subject(s) - gene isoform , fetus , troponin , heart development , medicine , messenger rna , troponin t , biology , endocrinology , troponin i , antibody , skeletal muscle , biochemistry , pregnancy , immunology , genetics , gene , embryonic stem cell , myocardial infarction
We have used antibodies specific for troponin I proteins to examine human cardiac development and have detected a transiently expressed developmental isoform. This isoform is distinct from adult cardiac troponin I (TnIc) but is indistinguishable, on the basis of electrophoretic mobility and antibody reactivity, from the isoform found in slow skeletal muscle (TnIs). Furthermore, we show that mRNA for TnIs is present in fetal. but not adult, heart. Analysis of a developmental series of fetal samples indicates that there is a transition in expression from TnIs to TnIc which occurs between 20 weeks fetal and 9 months postnatal development.