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Characterization and regulation of the expression of scyllatoxin (Leiurotoxin I) receptors in the human neuroblastoma cell line NB‐OK 1
Author(s) -
Gossen Denis,
Gesquière Jean-Claude,
Tastenoy Michèle,
Neef Philippe De,
Waelbroeck Magali,
Christophe Jean
Publication year - 1991
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(91)80815-k
Subject(s) - neuroblastoma , receptor , characterization (materials science) , cell culture , microbiology and biotechnology , chemistry , biology , biochemistry , genetics , nanotechnology , materials science
125 I‐[Tyr 2 ]scyllatoxin allowed to label a single class of high‐affinity receptors in membranes from the human neuroblastoma cell line NB‐OK 1. The K d of these receptors was 60 pM for scyllatoxin (Leiurotoxin I) and 20 pM for apamin and the B max was low (3.8 fmol/mg membrane protein). K + increased toxin binding at low concentrations but exerted opposite effects at high concentrations. Ca 2+ , guanidinium and Na + exerted only inhibitory effects on binding. Scyllatoxin binding sites were overexpressed 2.5‐fold after a 24‐h cell pretreatment with 2 mM butyrate. This effect was suppressed by cycloheximide.